Excitatory synaptic transmission in the central nervous system is mainly mediated by AMPA type glutamate receptors. AMPA receptors form tetrameric heteromers of subunits. Subunits can be classified into two groups based on the structure of their cytoplasmic tail. GluR1, GluR4 and GluR2long (an alternate splice form of GluR2) possess long cytoplasmic tails while GluR2 and 3 have short cytoplasmic tail. There is considerable evidence that synaptic insertion of GluR1-containing AMPA receptors contributes to the synaptic strengthening observed during LTP (Long term potentiation) in vitro and experience in vivo. Our main focus is to elucidate how environments modify synaptic delivery of AMPA receptors in vivo. We combine molecular biology, electrophysiology, imaging technique (in vivo imaging with two photon laser scanning microscope) together with proteomics.