• 横浜市立大学大学院・医学研究科 生理学














    教室員の紹介

    竹本 研 (たけもと きわむ)

    2009年10月-2015年3月 JSTさきがけ・脳情報の解読と制御領域(川人光男総括) 研究員兼任



    (研究テーマ)
    1. エオシンによる高効率CALI法を用いた、神経伝達物質受容体の光不活化技術の開発
    2. 内在性たんぱく質を網羅的に光不活化可能な新技術の開発
    3. 二光子顕微鏡による可視化と光操作を用いた記憶痕跡形成メカニズムの解明


    (全業績リスト)
    17. Takemoto K*, Iwanari H, Tada H, Suyama K, Sano A, Nagai T, Hamakubo T, and Takahashi T.*
    "Optical inactivation of synaptic AMPA receptors erases fear memory"
    Nat. Biotechnol., 35(1);38-47, 2017 (*co-corresponding author)
    See also NEWS AND VIEWS

    16. Tada H, Miyazaki T,
    Takemoto K, Takase K, Jitsuki S, Nakajima W, Koide M, Yamamoto N, Komiya K, Suyama K, Sano A, Taguchi A, and Takahashi T
    "Neonatal isolation augments social dominance by altering actin dynamics in the medial prefrontal cortex"
    Proc Natl Acad Sci U S A.,1606351113, 2016

    15. Sakamaki K, Ishii MT, Sakata T,
    Takemoto K, Takagi C, Takeuchi A, Morishita R, Takahashi H, Nozawa A, Shinoda H, Chiba K, Sugimoto H, Saito A, Tamate S, Satou Y, Sang-Kee J, Matsuoka S, Koyamada K, Sawasaki T, Nagai T, Ueno N
    "Dysregulation of a potassium channel, THIK-1, targeted by caspase-8 accelerates cell shrinkage"
    Biochem.Biophys.Acta., 1863(11);2766-2783, 2016

    14. Jitsuki S, Nakajima W, Takemoto K , Sano A, Tada H, Takahashi-Jitsuki A, Takahashi T.
    "Nogo Receptor Signaling Restricts Adult Neural Plasticity by Limiting Synaptic AMPA Receptor Delivery"
    Cerebral Cortex, 26(1); 427-439, 2016

    13. Liu T, Yamaguchi Y, Shirasaki Y, Shikada K, Hoshino K, Kaisho T, Takemoto K , Suzuki T, Kuranaga E, Ohara O, Miura M.
    "Single-cell imaging of caspase-1 dynamics reveals an all-or none inflammasome signaling responce"
    Cell Rep.8(4):974-982, 2014.

    12. Yamaguchi Y, Kuranaga E, Nakajima Y, Koto A, Takemoto K, Miura M.
    "In vivo monitoring of caspase activation using a fluorescence resonance energy transfer-based fluorescent probe"
    Methods Enzymol., 544; 299-325, 2014.

    11. Takemoto K , Matsuda T, Sakai N, Fu D, Noda M, Uchiyama S, Kotera I, Arai Y, Horiuchi M, Fukui K, Ayabe T, Inagaki F, Suzuki H, Nagai T.
    "SuperNova, a monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation"
    Sci. Rep., 3; 2629, 2013.

    10. Ohtsuka K, Sato S, Sato Y, Sota K, Ohzawa S, Matsuda T, Takemoto K, Takamune N, Juskowiak B, Nagai T, Takenaka S
    "Fluorescence imaging of potassium ions in living cells using a fluorescent probe based a thrombin binding aptamer-peptide conjyugate"
    Chem. Commun. 48(39):4740-2, 2012.

    9. Yamaguchi Y, Shinotsuka N, Nonomura K, Takemoto K , Kuida K, Yoshida H, Miura M.
    “Live imaging of apoptosis in a novel transgenic mouse highlights its role in neural tube closure“
    J.Cell.Biol. 195(6): 1047-60, 2011.

    8. Takemoto K , Matsuda T, McDougall M, Klaubert DH, Hasegawa A, Los GV, Wood KV, Miyawaki A, Nagai T.
    “Chromophore-assisted light inactivation of HaloTag fusion proteins labeled with eosin in living cells“
    ACS Chem Biol. 6(5): 401-6, 2011.

    7. Kuranaga E, Matsunuma T, Kanuka H, Takemoto K , Koto A, Kimura K, Miura M.
    "Apotosis controls the speed of looping morphogenesis in Drosophila male terminalia"
    Development. 138(8): 1493-9, 2011.

    6. Jitsuki S, Takemoto K , Kawasaki T, Tada H, Takahashi A, Becamel C, Sano A, Yuzaki M, Zukin RS, Ziff EB, Kessels HW, and Takahashi T.
    "Serotonin mediates cross-modal reorganization of cortical circuits".
    Neuron, 69, 780-792, 2011.

    5. Takemoto K , Kuranaga E, Tonoki A, Nagai T, Miyawaki A and Miura M.
    “Local initiation of caspase activation in Drosophila salivary gland programmed cell death in vivo “
    Proc Natl Acad Sci U S A. 104(33): 13367-13372, 2007.
     

    4. Kuranaga E, Kanuka H, Tonoki A, Takemoto K , Tomioka T, Kobayashi M, Hayashi S and Miura M.
    “Drosophila IKK-Related Kinase regulates non-apoptotic function of caspases via degradation of IAPs”
    Cell 126(3): 583-596, 2006.

    3. Kanuka H., Kuranaga E., Takemoto K ., Hiratou T., Okano H., and Miura M.
    “Drosophila caspase transduces Shaggy/GSK-3b kinase activity in neural precuorsor development.”
    EMBO J. 24(21): 3793-3806, 2005.

    2.Takemoto K , Nagai T, Miyawaki A, and Miura M.
    “Spatio-temporal activation of caspase revealed by indicator that is insensitive to environmental effects”
    J.Cell Biol. 160(2): 235-243, 2003.
    See also In This Issue.

    1. Uetsuki T, Takemoto K , Nishimura I, Okamoto M, Niinobe M, Momoi T, Miura M and Yoshikawa K.
    “Activation of Neuronal Caspase-3 by Intracellular Accumulation of Wild-Type Alzheimer Amyloid Precursor Protein”
    J.Neurosci. 19(16): 6955-6964, 1999.